Electrophoretic Variation of Creatine Kinase Isozymes as a Function of Methyl Parathion in the fish Labeo Rohita

Serum samples were obtained from the control and experimental fish, Labeo rohita treated with 1/3 of LC50 of methyl parathion. Creatine kinase (EC 2.7.3.2) isozyme and subbands (isoforms) were studied using clinical automatic electrophoresis and densitometer. CK isozymes and isoforms were found to display disparate electrophoretic mobility in fish when compared with CK patterns of human. It was interesting to observe that CK-MM was 90.1% in human while 75% in fish. CK-MB isozyme was present in human but absent in fish. Following the treatment of methyl parathion there was a marked increase in the CKactivity. The study reveals that methyl parathion exposure altered CK-B1 status in the brain due to induction of lipid peroxidation/ inhibition of acetylcholinesterase. The physiological and biochemical significance is reported herein for the first time.